Plasmid

Part:BBa_K112248:Design

Designed by: Molly Allen   Group: iGEM08_UC_Berkeley   (2008-10-28)


{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112950 plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3066
    Illegal XbaI site found at 4309
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3066
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 3066
    Illegal BamHI site found at 3976
    Illegal XhoI site found at 5462
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3066
    Illegal XbaI site found at 4309
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3066
    Illegal XbaI site found at 4309
    Illegal NgoMIV site found at 5315
    Illegal AgeI site found at 3714
    Illegal AgeI site found at 3826
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

?


Source

pAH57 plasmid

References